| Callsign | Network | Channel | City |
|---|---|---|---|
| KCWI | CW +6 | 23 | Ames |
| WOI | ABC +7 | 5 | Ames |
| KGCW | CW +3 | 26 | Burlington |
| KGAN | CBS +2 | 2 | Cedar Rapids |
| KFXA | Dabl +4 | 28 | Cedar Rapids |
| KCRG | ABC +2 | 9 | Cedar Rapids |
| KPXR | Ion +8 | 48 | Cedar Rapids |
| KBIN | PBS +3 | 32 | Council Bluffs |
| KQIN | PBS +3 | 36 | Davenport |
| KLJB | FOX +3 | 18 | Davenport |
| KWQC | NBC +6 | 6 | Davenport |
| KDMI | TCT +5 | 19 | Des Moines |
| KDIN | PBS +3 | 11 | Des Moines |
| KCCI | CBS +3 | 8 | Des Moines |
| WHO | NBC +3 | 13 | Des Moines |
| WBXF | The Country Network +3 | 28 | Des Moines |
| K32NM-D | HSN +1 | 32 | Des Moines |
| KRPG | Azteca America +2 | 43 | Des Moines |
| KDSM | FOX +3 | 17 | Des Moines |
| KFXB | CTN +1 | 40 | Dubuque |
| KAJR | Movies! +6 | 36 | Fort Dodge |
| KCYM | Newsmax +6 | 45 | Fort Dodge |
| KTIN | PBS +3 | 21 | Fort Dodge |
| KIIN | PBS +3 | 12 | Iowa City |
| KWKB | TCT +5 | 20 | Iowa City |
| KDIT | Decades +2 | 45 | Marshalltown |
| KIMT | CBS +3 | 3 | Mason City |
| KYIN | PBS +3 | 24 | Mason City |
| KFPX | Ion +7 | 39 | Newton |
| KYOU | FOX +5 | 15 | Ottumwa |
| KHIN | PBS +3 | 36 | Red Oak |
| KTIV | NBC +4 | 4 | Sioux City |
| KSIN | PBS +3 | 27 | Sioux City |
| KPTH | FOX +2 | 44 | Sioux City |
| KMEG | Dabl +3 | 14 | Sioux City |
| KCAU | ABC +3 | 9 | Sioux City |
| KWWL | NBC +4 | 7 | Waterloo |
| KRIN | PBS +3 | 32 | Waterloo |
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